Journal: Indian Journal of Pharmacology

Article Title: Platycodon grandiflorus alleviates DNCB-induced atopy-like dermatitis in NC/Nga mice

doi: 10.4103/0253-7613.99306

Figure Lengend Snippet: Effect of PG extracts for Total IgE levels in serum and infiltrated the mast cells in the DNCB-induced AD-like skin lesion (A) IgE concentration of each mouse was measured after sacrifice by ELISA. Data are presented as mean ± SEM (n = 5). Normalcontrol (NC), postive control (PC), PG1 (DNCB + PG 300 mg/kg), PG2 (DNCB + PG 500 mg/kg), DEX (DNCB + dexamethasone 1.5 mg/kg). (B) The number of infiltrating mast cells in the tissue sections of the skin lesion was counted in five sites chosen randomly. PG (DNCB + PG 500 mg/ kg). * P < 0.05 vs . PC.

Article Snippet: The levels of total serum IgE were measured by a mouse IgE ELISA kit (Shibayagi, Japan), following the manufacturer's instructions.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: Food & Nutrition Research

Article Title: A multistrain probiotic formulation attenuates skin symptoms of atopic dermatitis in a mouse model through the generation of CD4 + Foxp3 + T cells

doi: 10.3402/fnr.v60.32550

Figure Lengend Snippet: Serum IgE and inflammatory cytokine levels. Blood was obtained from NC/Nga mice at week 12 for which the IgE (a), IL-4 (b), IL-10 (c), IL-13 (d), IFN-γ (e), and IL-12p40 (f) serum levels were determined using ELISA. Results are expressed as the mean+SD ( n =12). ** p <0.01, *** p <0.001 versus the control group; ### p <0.001 versus the excipient group; §§§ p <0.001 versus the GI7-L group.

Article Snippet: IgE levels were determined using a mouse IgE ELISA kit (BD Biosciences, Franklin Lakes, NJ, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: MethodsX

Article Title: Development and evaluation of mouse anti-Ara h 1 and Ara h 3 IgE monoclonal antibodies for advancing peanut allergy research

doi: 10.1016/j.mex.2023.102470

Figure Lengend Snippet: Dose dependency of each IgE monoclonal antibody against CPE in a sandwich ELISA.

Article Snippet: All plates were screened for anti-CPE IgE antibody-producing hybridomas by ELISA kits (Cat# 3063, Chondrex, Inc. WA, USA), according to the manufacturer's instructions.

Techniques: Sandwich ELISA

Journal: MethodsX

Article Title: Development and evaluation of mouse anti-Ara h 1 and Ara h 3 IgE monoclonal antibodies for advancing peanut allergy research

doi: 10.1016/j.mex.2023.102470

Figure Lengend Snippet: Dose dependency of each IgE monoclonal antibody against CPE in an indirect ELISA.

Article Snippet: All plates were screened for anti-CPE IgE antibody-producing hybridomas by ELISA kits (Cat# 3063, Chondrex, Inc. WA, USA), according to the manufacturer's instructions.

Techniques: Indirect ELISA

Journal: MethodsX

Article Title: Development and evaluation of mouse anti-Ara h 1 and Ara h 3 IgE monoclonal antibodies for advancing peanut allergy research

doi: 10.1016/j.mex.2023.102470

Figure Lengend Snippet: Summary of the Application of Anti-CPE IgE Monoclonal Antibodies.

Article Snippet: All plates were screened for anti-CPE IgE antibody-producing hybridomas by ELISA kits (Cat# 3063, Chondrex, Inc. WA, USA), according to the manufacturer's instructions.

Techniques: Indirect ELISA, Sandwich ELISA